InSolution Caspase-3 Inhibitor I; Cell-Permeable

Biochem/physiol Actions

Reversible: yes

Primary Targetcaspase-3

Product does not compete with ATP.

Cell permeable: yes

Target IC₅₀: 200 pM inhibiting PARP cleavage in cultured human osteosarcoma cell extracts

General description

A cell-permeable inhibitor of caspase-3. The C-terminal DEVD-CHO sequence of this peptide is a highly specific, potent, and reversible inhibitor of caspase-3 (K_i﹤1 nM) that has also been shown to strongly inhibit PARP cleavage in cultured human osteosarcoma cell extracts (IC₅₀ = 200 pM). The N-terminal sequence (residues 1-16) corresponds to the hydrophobic region (h-region) of the signal peptide of Kaposi fibroblast growth factor (K-FGF) and confers cell-permeability to the peptide.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Lin, Y.-Z., et al. 1996. J. Biol. Chem. 271, 5305.Nicholson, D.W. 1996. Nature Biotechnol. 14, 297.Schlegel, J., et al. 1996. J. Biol. Chem.271, 1841.Lin, Y.-Z., et al. 1995. J. Biol. Chem.270, 14255.Nicholson, D.W., et al. 1995. Nature 376, 37.Tewari, M., et al. 1995. Cell 81, 801.Lazebnik, Y.A., et al. 1994. Nature371, 346.

Packaging

Packaged under inert gas

1 mg in Glass bottle

Physical form

A 5 mM (1 mg/100 µl) solution of Caspase-3 Inhibitor I, Cell-permeable (Cat. No. 235423) in DMSO.

Reconstitution

Following initial thaw, aliquot, purge with inert gas, and freeze (-20°C).

Sequence

Ac-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Asp-Glu-Val-Asp-CHO

Warning

Toxicity: Irritant (B)